Pengaruh Ekstrak Minyak Ikan Patin Terhadap Kadar Kolesterol Hdl Rattus Norvegicus yang Diinduksi Aloksan
Article Sidebar
Main Article Content
Abstract
Diabetes mellitus is a chronic condition due to insufficient amount of insulin produced by the pancreas. This condition can cause dyslipidemia. This study aimed to observe the effect of catfish (Pangasius sp) fish oil extract on HDL cholesterol level in male white rat (Rattus norvegicus) with alloxan-induced diabetes model. Alloxan was used to create diabetic conditions in the rats and the administration of catfish oil extract which contain omega-3 was expected to increase HDL cholesterol level.
This is an experimental laboratory study carried out in the Biochemistry Laboratory Hang Tuah Medical Faculty from the beginning of May 2021 to the end of December 2021.
Twenty seven male white rats selected based on the simple random sampling method divided into 3 groups: the negative control group with standard feeding and PDAM drinking water, the positive control group induced by alloxan 150 mg/kgBW, and the treatment group induced by alloxan 150 mg/kgBW and 3 days later given catfish (Pangasius sp) fish oil extract 73 mg/kgBW for 14 days. At the end of the study HDL cholesterol levels were measured in all sample.
Data analysis showed lower HDL cholesterol levels in the positive control group (mean=20.78 mg/dl) than in the negative control group (mean=31.00 mg/dl) significantly (p = 0.004) and higher HDL cholesterol levels in the treatment group (mean=27.00 mg/dl) than in the positive control group (mean=20.78 mg/dl) athough the increase was not significant (p = 0.91).
Conclusion of this study: the administration of catfish fish oil extract (Pangasius sp) dose of 73 mg/kgBW in alloxan induced male white rats (Rattus norvegicus) wistar strain increase HDL cholesterol levels although not significantly.
Keywords : Pangasius sp, HDL Cholesterol level, Rattus norvegicus, Alloxan.
Article Details
References
Ahyar, H. et al. (2020) Buku Metode Penelitian Kualitatif & Kuantitatif.
Bardini, G., Rotella, C. M. and Giannini, S. (2012) ‘Dyslipidemia and diabetes: reciprocal impact of impaired lipid metabolism and Beta-cell dysfunction on micro- and macrovascular complications.’, The review of diabetic studies : RDS. doi: 10.1900/RDS.2012.9.82.
Barter, P. J. (2011) ‘The causes and consequences of low levels of high density lipoproteins in patients with diabetes’, Diabetes and Metabolism Journal. doi: 10.4093/dmj.2011.35.2.101.
Chiesa, S. T. and Charakida, M. (2019) ‘High-Density Lipoprotein Function and Dysfunction in Health and Disease’, Cardiovascular Drugs and Therapy, 33(2), pp. 207–219. doi: 10.1007/s10557-018-06846-w.
Elbossaty, W. F. (2018) ‘Clinical Influence of Triple Omega Fatty Acids (Omega-3, 6, 9)’, Biomedical Journal of Scientific & Technical Research, 6(4), pp. 5332–5334. doi: 10.26717/bjstr.2018.06.001383.
European Society of Cardiology (2021) ‘Body mass index is a more powerful risk factor for diabetes than genetics -- ScienceDaily’, pp. 2020–2021.
Farbstein, D. and Levy, A. P. (2012) ‘HDL dysfunction in diabetes: Causes and possible treatments’, Expert Review of Cardiovascular Therapy. doi: 10.1586/erc.11.182.
Femlak, M. et al. (2017) ‘The role and function of HDL in patients with diabetes mellitus and the related cardiovascular risk’, Lipids in Health and Disease. doi: 10.1186/s12944-017-0594-3.
Galicia-Garcia, U. et al. (2020) ‘Pathophysiology of Type 2 Diabetes Mellitus.’, International journal of molecular sciences, 21(17), p. 6275. doi: 10.3390/ijms21176275.
Gordon, S. M. et al. (2011) ‘High density lipoprotein: It’s not just about lipid transport anymore’, Trends in Endocrinology and Metabolism. doi: 10.1016/j.tem.2010.10.001.
Gurr, M. I. et al. (2016) Lipids: Biochemistry, Biotechnology and Health.
Handajani, F. (2021) Metode Pemilihan Dan Pembuatan Hewan Model Beberapa Penyakit Pada Penelitian Eksperimental. Sidoarjo: Zifatama Jawara.
Hastarini, E. (2013) ‘Karakteristik Minyak Ikan Patin Dari Limbah Pengolahan Filet Ikan Patin Siam (Pangasius hypopthalmus) dan Patin Jambal(Pangasius djambal)’, Jurnal Agritech. doi: 10.22146/agritech.9584.
Hendra, R. D. (2015) Profil Lipid Darah Pada Model Tikus Pulpitis Dengan Paparan Streptoccocus Mutans, Digital Repository Universitas Jember. Availableat:http://repository.unej.ac.id/bitstream/handle/123456789/65672/Ainul Latifah-101810401034.pdf?sequence=1.
Hidayaturrahmah; Muhamat; Akbar, A. (2016) ‘Efek Ekstrak Minyak Ikan Patin (Pangasius hypopthalmus) Terhadap Peningkatan Memori dan Fungsi Kognitif Mencit Berdasarkan Passive Avoidance Test’, Jurnal Pharmascience, 03(02), pp. 14–22.
Hidayaturrahmah, H., Santoso, H. B. and Nurlely, N. (2017) ‘Profil Glukosa Darah Tikus Putih Setelah Pemberian Ekstrak Minyak Ikan Patin (Pangasius hypopthalmus) Sebagai Alternatif Antidiabetes’, Jurnal Pharmascience, 4(2), pp. 219–226. doi: 10.20527/jps.v4i2.5775.
Hod, M. et al. (2015) ‘ The International Federation of Gynecology and Obstetrics (FIGO) Initiative on gestational diabetes mellitus: A pragmatic guide for diagnosis, management, and care # ’, International Journal of Gynecology & Obstetrics. doi: 10.1016/s0020-7292(15)30033-3.
IDF Diabetes Atlas 9th edition (2019) IDF Diabetes Atlas 9th edition 2019, International Diabetes Federation Diabetes Atlas, Ninth Edition.
Ighodaro, O. M., Adeosun, A. M. and Akinloye, O. A. (2017) ‘Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies’, Medicina (Lithuania). doi: 10.1016/j.medici.2018.02.001.
Johnson, M. and Bradford, C. (2014) ‘Omega-3, Omega-6 and Omega-9 Fatty Acids: Implications for Cardiovascular and Other Diseases’, Journal of Glycomics & Lipidomics, 04(04), pp. 4–11. doi: 10.4172/2153-0637.1000123.
Lestari, D. U., Sumardianto, S. and Purnamayati, L. (2020) ‘The Characteristics of Striped Catfish Oil (Pangasius hypophthalmus) Extracted by Dry Rendering Method at Different Temperatures’, Caraka Tani: Journal of Sustainable Agriculture. doi: 10.20961/carakatani.v35i1.31604.
M.Harmain, R. and Dali, F. A. (2017) ‘Buku Ajar Ilabulo Ikan Patin Pangasius’, p. 52.
Murray, R. K. et al. (2018) Harper’s Illustrated Biochemistry (31st Edition), Biochemical Education.
Parhofer, K. G. (2011) ‘Pathophysiology of diabetic dyslipidemia: Implications for atherogenesis and treatment’, Clinical Lipidology, 6(4), pp. 401–411. doi: 10.2217/clp.11.32.
PERKENI (2019) Pedoman pengelolaan dan pencegahan diabetes melitus tipe 2 di Indonesia, Perkeni.
Punthakee, Z., Goldenberg, R. and Katz, P. (2018) ‘Definition, Classification and Diagnosis of Diabetes, Prediabetes and Metabolic Syndrome’, Canadian Journal of Diabetes, 42, pp. S10–S15. doi: 10.1016/j.jcjd.2017.10.003.
Ridwan, E. (2013) ‘Etika Pemanfaatan Hewan Percobaan dalam Penelitian Kesehatan Ethical Use of Animals in Medical Research’, J Indon Med Assoc, 63(3),pp.112–116.Availableat: http://docshare01.docshare.tips/files/30994/309947046.pdf.
Rondanelli, M. et al. (2016) ‘MediterrAsian diet products that could raise HDL-cholesterol: A systematic review’, BioMed Research International. doi: 10.1155/2016/2025687.
Sastroasmoro, S. (2011) ‘Perkiraan Besar Sampel dalam Penelitian Klinis’, Dasar-dasar Metodologi Penelitian, p. 359.
Satrianawaty, L. D., Martini, T. and Prabowo, S. (2019) ‘Pengaruh Pemberian Ekstrak Daun Kelor terhadap Kadar Kolesterol HDL Tikus Putih Jantan Hiperglikemia dengan Induksi Aloksan’, Hang Tuah Medical journal. doi: 10.30649/htmj.v17i1.121.
Sheriff, O. L. et al. (2020) ‘A New model for Alloxan-induced diabetes mellitus in rats’, Journal of Bangladesh Society of Physiologist, 14(2), pp. 56–62. doi: 10.3329/jbsp.v14i2.44785.
Soliman, M. M. et al. (2020) ‘High density lipoprotein (HDL) level may be a risk factor of lower extremities amputation (LEA) in patients with diabetic foot ulcer (DFU)’, Research Journal of Pharmaceutical, Biological and Chemical Sciences, 11(2), pp. 158–169. doi: 10.33887/rjpbcs/2020.11.
Tyastirin, E. and Hidayati, I. (2017) Statistik parametrik untuk penelitian kesehatan, Program Studi Arsitektur Uin Sunan Ampel.
Wardeh, Y. S. A., Al-Saqri, S. and AL-Nasr, T. (2016) ‘The Most Significant Modifiable and non-Modifiable Factors Associated among Patients Whom Previously Diagnosed with Prediabetes and Developed to Type Two Diabetes Mellitus Attending to Endocrine Clinic in all Hail Governmental Hospitals’, 31, pp. 39–49.
World Health Organization (2016) ‘Global Report on Diabetes’, Isbn. doi: ISBN 978 92 4 156525 7.
Wu, L. and Parhofer, K. G. (2014) ‘Diabetic dyslipidemia’, Metabolism: Clinical and Experimental. doi: 10.1016/j.metabol.2014.08.010.
Yanai, H. et al. (2018) ‘An Improvement of Cardiovascular Risk Factors by Omega-3 Polyunsaturated Fatty Acids’, Journal of Clinical Medicine Research, 10(4), pp. 281–289. doi: 10.14740/jocmr3362w.
Zhang, J. et al. (2015) ‘Association between family history risk categories and prevalence of diabetes in chinese population’, PLoS ONE, 10(2), pp. 1–13. doi: 10.1371/journal.pone.0117044.