PENGARUH EKSTRAK KULIT BATANG Rhizophora apiculata TERHADAP KADAR GLUKOSA DARAH Rattus norvegicus YANG DIINDUKSI DEKSAMETASON

ARLISA  NABILA LARASATI; FITRI HANDAJANI; SAPTONO  PUTRO; INTAN  KOMALASARI

doi:10.30649/htmj.v23i2.874

Authors

ARLISA NABILA LARASATI Fakultas Kedokteran, Universitas Hang Tuah
FITRI HANDAJANI
SAPTONO PUTRO Fakultas Kedokteran, Universitas Hang Tuah
INTAN KOMALASARI Fakultas Kedokteran, Universitas Hang Tuah

DOI:

https://doi.org/10.30649/htmj.v23i2.874

Keywords:

dexamethasone, Rhizophora apiculata, Rattus norvegicus, blood glucose,, hyperglycemia

Abstract

Background: Dexamethasone is a glucocorticoid, if used long term it can cause increased oxidative stress and hyperglycemia. In diabetes patients, large amounts of antioxidants are needed as an antidote to free radicals in the body. Ethanol extract of Rhizophora apiculata stem bark has antioxidant and antidiabetic activity.

Objective: This study aims to evaluate the effect of ethanol extract of Rhizophora apiculata bark on the blood glucose levels of white rats induced by dexamethasone.

Methods: This research use 32 rats grouped into 4 groups. G (-), standard feed, G (+) induced by dexamethasone, GP 1 bark extraxt and induced by dexamethasone at the same time as giving mangrove bark extract, and DT 2, dexamethasone and mangrove bark extract, then on the 22nd day a blood sample was taken to measure blood glucose levels.

Results: the average blood glucose level G- = 146.5; G+= 213.375; GP = 185.875; GT = 244.375. In the One Way Anova parametric test, significant results were obtained, 0.015 (p<0.05). Then in the Post Hoc LSD test; G- and G+ had a significance of 0.028 (p<0.05), while the G+ and GP and GT groups did not show significant differences.

Conclusion: Subcutaneous dexamethasone induction treatment was proven to increase blood glucose levels in male Rattus norvegicus. Administration of ethanol extract of Rhizophora apiculata stem bark as prophylaxis or therapy was unable to reduce blood glucose levels of male Rattus norvegicus induced by dexamethasone.

Author Biographies

SAPTONO PUTRO, Fakultas Kedokteran, Universitas Hang Tuah

Departemen Kesehatan Kelautan dan Hiperbarik, Fakultas Kedokteran, Universitas Hang Tuah Surabaya

INTAN KOMALASARI, Fakultas Kedokteran, Universitas Hang Tuah

departemen ilmu penyakit jantung

References

Alfa, N., 2019. Pengaruh Pemberian Ekstrak Kulit Batang Bakau Minyak (Rhizophora apiculata) Etanol 95 % Terhadap Gambaran Histopatologi Arteri Koronaria Tikus Putih (Rattus novergicus) Jantan Galur Sprague Dawley Yang Dipaparkan Asap Rokok. Skripsi. Universitas Lampung. [online]

Alves, J.D., Suwarman, S. and Kestriani, N.D., 2019. Pengaruh Deksametason 0,2 mg/KgBB Sebagai Adjuvan Analgesia terhadap Waktu Kejadian Nyeri Pascaoperasi Odontektomi dengan Nrs >3. Jurnal Anestesi Perioperatif, 7(3), pp.168–174. https://doi.org/10.15851/jap.v7n3.1826.

Askar, M., E-H Ibrahim, I. and Mahmoud, M., 2022. Dexamethasone-Induced Metabolic Syndrome: Re-Evaluation Of An Underestimated Experimental Model. Bulletin of Pharmaceutical Sciences. Assiut, 45(2), pp.989–1003. https://doi.org/10.21608/bfsa.2022.271784.

Ayu Wardina, M., Mustofa, S., Nafisah Tendri Adjeng Malarangeng, A.,. Review Article: Potensi Rhizophora apiculata Sebagai Fitofarmaka. Biokimia Biologi Molekular dan Fisiologi, B. and Farmasi, B., 2023. [online] Available at: <https://www.journalofmedula.com/index.php/medula/article/view/620/47> [Accessed 11 June 2024].

Bintang Bella Pertiwi, M., Erma Indahyani, D., Praharani, D., 2021. Level Glukosa Darah pada Mencit Diabetes Setelah Pemberian Ekstrak Rumput Laut Coklat (Phaeophyta) (Blood Glucose Levels in Diabetic Mice After Giving Brown Seaweed (Phaeophyta) Extract). Level Glukosa Darah pada Mencit Diabetes Setelah Pemberian Ekstrak Rumput Laut Coklat. e-Journal Pustaka Kesehatan,. [online]

Dat, T.T.H., Oanh, P.T.T., Cuong, L.C.V., Anh, L.T., Minh, L.T.H., Ha, H., et al, 2021. Pharmacological Properties, Volatile Organic Compounds, and Genome Sequences of Bacterial Endophytes from the Mangrove Plant Rhizophora apiculata Blume. Antibiotics (Basel, Switzerland), 10(12). https://doi.org/10.3390/antibiotics10121491.

Dewi, M.S., Tri Nuraini, R.A., Yulianto, B. and Sibero, M.T., 2023. Kandungan Senyawa Bioaktif dan Aktivitas Biologis Daun Mangrove Lumnitzera racemosa di Pantai Teluk Awur dan Pantai Blebak Jepara. Journal of Marine Research, 12(3), pp.391–402. https://doi.org/10.14710/jmr.v12i3.34584.

Handajani, F., 2021. Metode Pemilihan dan Pembuatan Hewan Coba.

Hanim, R., Darusman, H.S. and Rahminiwati, M., 2018. Studi Karakteristik Tipe Diabetes pada Tikus (Rattus novergicus) yang Diinduksi Deksametason (Characteristic Study Of The Type Of Diabetes In Rat (Rattus Novergicus) Model Induced By Dexamethasone). Jurnal Veteriner, 19(1), p.1. https://doi.org/10.19087/jveteriner.2018.19.1.1.

Hassan-Danboyi, E., Jimoh, A., Alhassan, A., Danboyi, T., Mohammed, K., Dubo, A., et al, 2021. Antioxidant effects of L-citrulline supplementation in high-fat diet- and dexamethasone-induced Type-2 diabetes mellitus in wistar rats (Rattus norvegicus). Nigerian Journal of Experimental and Clinical Biosciences, 9(2), p.95. https://doi.org/10.4103/njecp.njecp_4_21.

Imanda, Y.L., Zamhari, M. and Nurleni, N., 2023. Perbandingan Aktivitas Antidiabetes Produk Obat Herbal Jamu “X” Dan “Y” Yang Mengandung Ekstrak Mengkudu (Morinda citrifolia l.) Pada Mencit Jantan Model Diabetes Yang Diinduksi Dengan Deksametason Dan Dekstrosa 40%. Jurnal Ilmiah Bakti Farmasi, 7(1). https://doi.org/10.61685/jibf.v7i1.84.

Maharani, A.D.K., Santoso, P. and Udayani, N.N.W., 2023. Efek Ekstrak Etanol Buah Dewandaru (Eugenia uniflora L) terhadap Penurunan Kadar Glukosa Darah dan Perbaikan Sel Beta Pankreas pada Mencit yang Diinduksi Aloksan. Skripsi. Usadha, [online] 2(2), pp.39–44. https://doi.org/10.36733/usadha.v2i3.7384.

Mustofa, S. and Tarigan, C.Y., 2023. Efek Protektif Ekstrak Kulit Batang Bakau Rhizophora apiculata terhadap Kerusakan Histologi Paru Rattus norvegicus yang Diinduksi Asap Rokok. Jurnal Kesehatan, 14(2), p.241. https://doi.org/10.26630/jk.v14i2.3752.

Nkono, B.L.N.Y., Noa, P.Y.A., Kedi, L.M., Nzikoue, S. and Sokeng, S.D., 2023. Antihyperglycemic and antihyperlipidemic effects of Cymbopogon citratus leaves in dexamethasone-induced diabetic rats. International Journal of Science and Technology Research Archive, 4(2), pp.056–066. https://doi.org/10.53771/ijstra.2023.4.2.0060.

Ramadhan, W.I., 2019. Pengaruh Pemberian Seduhan Teh Hijau Daun Mangrove Bakau (Rhizopora Apiculata) Terhadap Kadar Glukosa Darah Tikus Wistar Putih (Rattus Novergicus) Diabetes Mellitus. Tesis. Universitas Brawijaya.

Rascón-Careaga, A., Corella-Madueño, M.A.G., Pérez-Martínez, C.J., García-Rojas, A.M., Souflé-Vásquez, S.Z., García-Moroyoqui, M.T., et al, 2021. Validation and Estimation of Uncertainty for a Glucose Determination Method GOD-PAP Using a Multi-calibrator as Reference. MAPAN, 36(2), pp.269–278. https://doi.org/10.1007/s12647-021-00441-5.

Selvaraj, G., Kaliamurthi, S. and Thirugnasambandan, R., 2016. Effect of Glycosin alkaloid from Rhizophora apiculata in non-insulin dependent diabetic rats and its mechanism of action: In vivo and in silico studies. Phytomedicine, 23(6), pp.632–640. https://doi.org/10.1016/j.phymed.2016.03.004.

Tarigan, P., 2022. Gambaran Kadar Glukosa Pada Wanita Menopause. [Online] Available at: <http://ecampus.poltekkesmedan. ac.id/xmlui/handle/123456789/6756>.

Timmermans, S., Souffriau, J. and Libert, C., 2019. A General Introduction to Glucocorticoid Biology. Frontiers in Immunology, 10. https://doi.org/10.3389/fimmu.2019.01545.

Zain, M. F., 2023 Pengaruh Pemberian Ekstrak Etanol 95% Kulit Batang Rhizophora apiculata Terhadap Kadar Plasma Enzim Alanin Aminotransferase (Alt) Dan Aspartat Aminotransferase (Ast) Tikus Putih Jantan (Rattus norvegicus) Galur Sprague Dawley Yang Diinduksi Parasetamol. Skripsi. Universitas Lampung. [online]